SPR

Surface Plasmon Resonance (SPR) is a label-free, real-time optical biosensor technology that measures changes in the refractive index near a sensor surface. By monitoring these changes, SPR provides detailed kinetic and affinity data on molecular interactions, such as protein-protein, protein-DNA, and protein-small molecule bindings. The technique is based on the excitation of surface plasmons when polarized light strikes a thin metal film under conditions of total internal reflection. As biomolecules bind to the sensor surface, the resonance angle shifts, producing a measurable signal that reflects the binding events. Widely used in drug discovery, diagnostics, and fundamental biochemical research, SPR offers high sensitivity and rapid analysis, making it an essential tool in understanding complex biological processes.