pAb Multi-species
Enhance the sensitivity of your immunoassays with InnoTrace's comprehensive polyclonal antibody production solutions. From antigen design to serum purification, our optimized workflow ensures high-quality antibodies with maximum yield. Generate high-affinity polyclonal antibodies using any antigen in a variety of species, including rabbit, chicken, mouse, rat, guinea pig, goat, sheep, llama, and alpaca!
InnoTrace Polyclonal Antibody Production offers custom antigen design, immunization, and high-yield antibody purification for various species, including rabbit, mouse, goat, sheep, llama, and alpaca. Our optimized workflow ensures high-affinity, high-specificity antibodies for research, diagnostics, and therapeutics.
InnoTrace specializes in producing polyclonal antibodies using various antigen types, including peptides, proteins, DNA, small molecules, and cell lysates. Our advanced facilities also support custom antigen production or the use of client-provided antigens.
Antigen Design and Production
Maximizing antibody specificity and immune response depends on selecting the right antigen. InnoTrace offers antigen generation for:
Proteins: The most common choice, ensuring broad epitope exposure.
Peptides: Custom-synthesized for linear epitopes, ideal for Western Blot and ELISA.
DNA: Used in special cases where traditional immunization is difficult.
Small Molecules: Requires conjugation with carrier proteins.
Animal Immunization
Our standard immunization protocols include:
51-day schedule for protein-based polyclonal antibody production.
70-day schedule for peptide-based polyclonal antibody production.
Extended immunization schedules are available for higher titers. Lower antigen doses with longer immunization periods generally yield the best results.
Polyclonal Antibody Purification
After reaching peak antibody titers, InnoTrace purifies polyclonal antibodies using:
Protein A/G Purification: High-yield purification with minimal non-specific binding.
Antigen-Specific Affinity Purification: Removes non-specific antibodies for maximum specificity.
We regularly test serum samples from hyperimmunized hosts using ELISA and, upon request, Western Blot for additional validation.
