InnoTrace Biotechnologies - Anti-small Molecules

Anti-small molecules

Anti-small molecule antibodies are tailored to specifically recognize haptens, including organic small molecules and short peptide chains with unique conformations. InnoTrace provides comprehensive customization services, covering small molecule synthesis and conjugation, animal immunization, antibody screening and purification, as well as recombinant expression and functional validation.

To meet diverse research needs, InnoTrace employs advanced platforms such as the Single B Rabbit Monoclonal Antibody Screening Platform, Mouse Hybridoma Monoclonal Antibody Screening Platform, Rat/Rabbit Polyclonal Antibody Screening Platform, and the Phage Display Antibody Library Platform, ensuring high-quality, precise solutions.

The development of small-molecule antibodies begins by conjugating non-immunogenic small molecules with immunogenic protein carriers to create antigenic conjugates. This process enables immune recognition, laying the foundation for high-quality antibody production.

Through immunization, mice produce monoclonal antibodies with high specificity, while rabbits generate polyclonal antibodies with broad epitope coverage. This streamlined approach ensures versatile antibody solutions for research, diagnostics, and therapeutic applications.

pAb Multi-species FAQs

Polyclonal Antibody Production FAQ | Antigen Selection & Purification

What types of antigens can be used for polyclonal antibody production?

InnoTrace specializes in producing polyclonal antibodies using various antigen types, including peptides, proteins, DNA, small molecules, and cell lysates. Our advanced facilities also support custom antigen production or the use of client-provided antigens.

How to optimize the polyclonal antibody production process?

Antigen Design and Production

Maximizing antibody specificity and immune response depends on selecting the right antigen. InnoTrace offers antigen generation for:

Proteins: The most common choice, ensuring broad epitope exposure.
Peptides: Custom-synthesized for linear epitopes, ideal for Western Blot and ELISA.
DNA: Used in special cases where traditional immunization is difficult.
Small Molecules: Requires conjugation with carrier proteins.

Animal Immunization

Our standard immunization protocols include:

51-day schedule for protein-based polyclonal antibody production.
70-day schedule for peptide-based polyclonal antibody production.

Extended immunization schedules are available for higher titers. Lower antigen doses with longer immunization periods generally yield the best results.

Polyclonal Antibody Purification

After reaching peak antibody titers, InnoTrace purifies polyclonal antibodies using:

Protein A/G Purification: High-yield purification with minimal non-specific binding.
Antigen-Specific Affinity Purification: Removes non-specific antibodies for maximum specificity.

How do you measure antibody titers?

We regularly test serum samples from hyperimmunized hosts using ELISA and, upon request, Western Blot for additional validation.

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