Product Details

Description

Recombinant human alpha A Crystallin/CRYAA protein is a Human Full Length protein, in the 1 to 173 aa range, expressed in Escherichia coli, with >96% purity and suitable for WB, FuncS, SDS-PAGE. 

Specification

Chemical

Concentration                                                   1 mg/mL, The concentration of this product may be batch-dependent 

Endotoxin                                                         1.0 EU per μg protein as determined by the LAL method 

Purity                                                                >96% SDS-PAGE; ≥ 98% as determined by SEC 

Biological

Application                                                        WB, FuncS, SDS-PAGE 

Biologically Active                                             Yes

Expression System                                           Escherichia coli

Organism                                                          Homo Sapiens, Human 

Protein Construction                                        CRYAA (Uniprot: P02489) expressed with C-terminal 6 x his tags 

Protein Type                                                     Recombinant 

Shipping and Handling

Shipping Condition                                            Blue ice 

Storage Buffer                                                   20 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA 

Storage Temperature                                        -80℃ for long term storage; avoid freeze / thaw cycle 

Applications

1. Cataract Research and Therapy
Gene Therapy: CRYAA is a target for gene therapy in treating congenital cataracts and other genetic disorders related to lens opacity. Gene therapy strategies aim to correct mutations in the CRYAA gene or to restore CRYAA function in individuals with impaired protein function to prevent or treat cataracts.
Protein Replacement Therapy: In cases where CRYAA is misfolded or dysfunctional due to genetic mutations, protein replacement therapies may be developed to provide functional CRYAA to the lens, potentially preventing the aggregation of proteins and cataract formation.
Cataractogenesis Studies: CRYAA is a central protein in cataract formation and is extensively studied to understand the underlying pathophysiology of cataracts and how mutations or dysfunctions in CRYAA contribute to protein aggregation in the lens.

2. Molecular Chaperone Studies
Protein Stability Research: CRYAA is an important small heat shock protein (sHSP) and is widely used in studies of protein stability, especially those focused on preventing protein misfolding and aggregation in various conditions. It helps in refolding denatured proteins and preventing aggregation under stress conditions, making it a key molecule in protein folding research.
Chaperone Activity Assays: Researchers use CRYAA in assays to assess molecular chaperone activity, as it helps to prevent the aggregation of other proteins, especially under conditions of heat or oxidative stress. It is often used as a model for studying the functions of other small heat shock proteins.

3. Neurodegenerative Disease Research
Alzheimer’s and Parkinson’s Disease: The properties of CRYAA as a chaperone protein are studied in the context of neurodegenerative diseases, where protein aggregation is a hallmark (e.g., amyloid-beta in Alzheimer's and alpha-synuclein in Parkinson's disease). CRYAA may be investigated for its potential to prevent protein aggregation in neuronal cells, offering possible therapeutic insights for diseases associated with protein misfolding.
Protein Misfolding Disorders: CRYAA’s molecular chaperone properties are also studied for their potential in the treatment of various protein misfolding disorders beyond cataracts, including diseases such as Huntington’s disease, prion diseases, and amyotrophic lateral sclerosis (ALS).

4. Diagnostic Applications
Biomarker for Cataracts: CRYAA expression levels or its mutation profile can serve as a biomarker for cataract diagnosis and for identifying individuals at high risk of cataract development, particularly in congenital or early-onset cases.
Genetic Screening: CRYAA mutations are associated with specific types of congenital cataracts. Therefore, genetic screening for CRYAA mutations can be used for early diagnosis and genetic counseling in families with a history of cataracts or other lens-related disorders.
Ocular Disease Research: CRYAA is also used in diagnostic assays to assess protein aggregation and lens health in the study of various ocular diseases beyond cataracts, such as lens opacification and presenile cataracts.

5. Protein Aggregation and Folding Studies
In Vitro Studies: CRYAA is frequently used in in vitro studies to investigate how proteins aggregate under stress conditions and how chaperones like CRYAA can assist in protein folding. This has important applications not only in cataract research but also in the broader field of protein chemistry.
Chaperone-Substrate Interactions: CRYAA’s interaction with unfolded or partially folded proteins makes it a critical tool in studying protein-substrate interactions. Researchers use CRYAA to examine the specificity and efficiency of chaperone-protein interactions.

6. Cryopreservation and Cell Preservation
Cryoprotectant Studies: CRYAA is involved in the maintenance of protein stability under stress, making it a candidate for research into cryopreservation and the preservation of cells, tissues, and organs. It is particularly useful in studies on freeze-thawing and other preservation techniques.
Cell Culture: CRYAA is used in cell culture models to help protect cells from oxidative stress and protein damage during experimental conditions that mimic stressful environments (e.g., hypoxia, hyperthermia).

7. Regenerative Medicine and Stem Cell Research
Stem Cell Protection: In stem cell therapies, CRYAA is used to protect stem cells from stress conditions, such as oxidative damage or thermal stress, during expansion or differentiation. Its molecular chaperone properties help in maintaining the integrity of stem cell proteins, ensuring that they maintain their pluripotency or differentiation potential.
Tissue Engineering: CRYAA is investigated in the context of tissue engineering and regenerative medicine, particularly for applications involving the lens, where its role in protein aggregation prevention can be critical for the regeneration of lens tissue.

8. Pharmaceutical and Biotechnology Applications
Therapeutic Development: As a molecular chaperone, CRYAA could potentially be used in the development of pharmaceuticals aimed at treating diseases related to protein aggregation or misfolding. Its ability to prevent protein aggregation makes it a potential therapeutic protein for diseases where protein misfolding is a key pathogenic mechanism.
Therapeutic Protein Formulations: CRYAA is also studied for use in biopharmaceutical formulations where it could be employed as an excipient or adjunct to prevent the aggregation of therapeutic proteins, especially in protein-based biologics.

9. Eye Tissue Engineering and Lens Regeneration
Lens Regeneration: CRYAA is of significant interest in ocular regenerative medicine for its potential to aid in the regeneration of lens tissue in conditions such as cataracts or lens damage due to trauma or disease. Research is ongoing to understand how CRYAA and other crystallins may contribute to the regeneration of lens cells and the restoration of lens transparency.

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