ExoPura® Basic Exosome Purification Column Kit-5T
ExoPura® Basic Exosome Purification Column Kit-5T
Product Name: Exosome Extraction and Purification Kit-5T
Catalog No.: INX-EXOPURA-CB-5T
Size: 5T
Sample Type: Biological Samples
Shipping temp: ambient
Storage: ambient
Trial Size: 1T/$458
Overview
ExoPura® Basic Exosome Purification Column Kit is designed for the isolation and purification of exosome vesicles from
highly clarified, non-complex biological samples (e.g., cell supernatant, clarified milk). The ExoPura® Basic column utilizes size
exclusion technology, separating particles based on size as they pass through a column filled with porous polysaccharide resin.
Under the influence of gravity, larger particles elute first, while smaller particles enter the resin pores during their descent,
delaying their elution. The column is composed of solid resin (stationary phase) and liquid buffer (mobile phase). No chemical
interactions occur between the particles and the resin, ensuring stability and reproducibility in separation.
This kit features rapid processing, high efficiency, and high-purity exosome recovery. It is suitable for various applications,
including electron microscopy, NTA particle size analysis, nucleic acid analysis, protein analysis, cell-based experiments, and
animal studies. Utilizing size exclusion technology, it is applicable to the separation and purification of exosome vesicles in non-
complex biological samples with high clarity (such as cell supernatants, clarified milk, etc.), and it is reusable.
Note: This kit is an exosome purification kit only and does not concentrate samples. It requires the use of an exosome extraction
kit or other methods for sample concentration pre-treatment.
Product Components
Component Name Spec
ExoPura® Basic Column 5T
Adapter 5T
Regeneration Solution 300 mL
Exosome Purification Filter 25T
Advantages of the Product
Ideal for exosome purification with significantly enhanced purity.
Reusable up to five times.
No ultracentrifugation required, preserving better exosome activity.
Rapid extraction, completed in just 30 minutes!
Data
Note: The blue column diagram represents the recommended collection fractions, divided into exosome fractions and three 0.5 mL fractions.
Case: ExoPura® Basic Purification of 293T Cell Supernatant (with 10% exosome-depleted FBS)
Sample Preparation: Utilize the Umibio Exosome Extraction and Concentration Kit (cell supernatant) to concentrate 50 mL of supernatant down to 0.5 mL. The prepared sample is then loaded onto the EPF column.
Purification Outcome: Collect the exosome-depleted fraction and recover 1.5 mL of purified exosome-rich fraction.
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